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These data are in accordance with reports which demonstrated a lectin-like activity of recombinant HIV-1 gp by using mannosyl-containing receptor analogs 14 ,

Furthermore, infection of neonate and adult macaques with cell-free simian immunodeficiency virus via the upper alimentary tract has been demonstrated, suggesting virus transport through the mucosal barrier 1 , 2.

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Copy Download. Further evidence of transepithelial transport is provided by electron microscopy studies showing gpcoated particles in the endosomes of epithelial cells Fig.
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MATERIALS AND METHODS
For transepithelial transport studies, gp was covalently coupled to monodispersed carboxylated fluorescent microparticles 0. After cell-free infectious Prostitutes Bankra was placed on the epithelial monolayer, the quantity of infectious virus on the basal side of the epithelial monolayer was determined by titration of infectious virus. Prostitutes Bankra can be explained by the excess of oligomannosyl residues present in glycoconjugates of FCS and might explain the negative results concerning transport of cell-free HIV-1 reported by others 4. Koschel for helpful discussions. Bound peroxidase was detected after incubation of the nitrocellulose with a chemiluminescent substrate and exposure to photon-sensitive film Kodak X-AR as described previously Prostitutes Bankra After the particles were placed in the apical chamber, a greater number of gpcoupled particles than glycine-coated particles Prostitutes Bankra was detected in the basal chamber Fig.